|Zhang, Xiaohui -|
|Jacob, Melissa -|
|Rao, Ranga -|
|Wang, Yanhong -|
|Agarwal, Ameeta -|
|Newman, David -|
|Khan, Ikhlas -|
|Clark, Alice -|
|Li, Xing-Cong -|
Submitted to: Journal of Medicinal Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 12, 2012
Publication Date: May 16, 2012
Citation: Zhang, X., Jacob, M.R., Rao, R.R., Wang, Y., Agarwal, A.K., Newman, D.J., Khan, I.A., Clark, A.M., Li, X. 2012. Antifungal cyclic peptides from the marine sponge Microscleroderma herdmani. Journal of Medicinal Chemistry. 2:7-14. Interpretive Summary: This manuscript describes the isolation, structure elucidation, and antifungal activity of cyclic peptides from the deep water sponge Microscleroderma herdmani.
Technical Abstract: Screening natural product extracts from National Cancer Institute Open Repository for antifungal discovery afforded hits for bioassay-guided fractionation. Upon LC-MS analysis of column fractions with antifungal activities to generate information on chemical structure, two new cyclic hexapeptides, microsclerodermins J (1) and K (2), were isolated from the deep water sponge Microscleroderma herdmani, along with microsclerodermins A (3) and B (4) previously isolated from an unidentified Microscleroderma species. The structures of the new compounds were elucidated by spectroscopic analysis and chemical methods. In vitro antifungal testing showed that the four compounds possess strong activities against the opportunistic fungal pathogens Candida albicans, Candida glabrata, Candida krusei, Cryptococcus neoformans, and Aspergillus fumigatus.