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Research Project:
IMPROVING BIOCHEMICAL PROCESSES FOR THE PRODUCTION OF SUSTAINABLE FUELS AND CHEMICALS
Location: Renewable Product Technology Research Unit
Title: UV-C mutagenesis of Kluyveromyces marxianus NRRL Y-1109 strain for improved anaerobic growth at elevated temperature on pentose and hexose sugars
Authors
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: April 21, 2012
Publication Date: April 21, 2012
Citation: Hughes, S.R., Rich, J.O. 2012. UV-C mutagenesis of Kluyveromyces marxianus NRRL Y-1109 strain for improved anaerobic growth at elevated temperature on pentose and hexose sugars [abstract]. American Chemical Society.
Technical Abstract:
More robust industrial yeast strains from Kluyveromyces marxianus NRRL Y-1109 and have been produced using UV-C irradiation specifically for anaerobic conversion of lignocellulosic sugar streams to fuel ethanol at elevated temperature (45°C). This type of random mutagenesis offers the possibility of generating large numbers of mutations broadly over the whole genome. When all of the genes were mutagenized to average 80% kill level and selected in sufficient numbers using automated plating strategies, we observed strains with improved utilization of sugars from lignocellulosic feedstocks under anaerobic conditions and at elevated temperatures.
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Last Modified: 05/20/2013
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