ARS | Publication request: SNP-based genotyping in lentil: linking sequence information with phenotypes

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: December 15, 2011
Publication Date: January 14, 2012
Citation: Bett, K., Sharpe, A., Coyne, C.J., Mcgee, R.J., Ramsay, L.D., Clarke, W.E., Sanderson, L., Fedoruk, M., Vandenberg, B. 2012. SNP-based genotyping in lentil: linking sequence information with phenotypes. Meeting Abstract. Plant and Animal Genome XX, San Diego, CA January 14-18, 2012.

Technical Abstract: Lentil (Lens culinaris) has been late to enter the world of high throughput molecular analysis due to a general lack of genomic resources. Using a 454 sequencing-based approach, SNPs have been identified in genes across the lentil genome. Several hundred have been turned into single SNP KASP assays (KBioscience) and a 1536-SNP Illumina Golden Gate assay has been developed. A collection of over 300 diverse lines has been genotyped to assess genetic diversity and structure in lentil germplasm. A gene-based map of the lentil genome has been developed that has been used to identify regions of conserved synteny with Medicago truncatula. Phenotypic evaluation of both bi-parental and association mapping populations is underway and is leading to the identification of both markers and genes associated with traits of interest to the lentil breeding program.