Technical Abstract: Epidemiologic studies correlate low vitamin C intake with bone loss. The genetic deletion of enzymes involved in de novo vitamin C synthesis in mice, likewise, causes severe osteoporosis. However, very few studies have evaluated a protective role of this dietary supplement on the skeleton. Here, we show that the ingestion of vitamin C reverses the low-turnover bone loss following ovariectomy in mice. This reversal, noted both on areal bone mineral density and µCT parameters, results primarily from the stimulation of bone formation, demonstrable in vivo by histomorphometry, bone marker measurements, and quantitative PCR. Notably, the reductions in the bone formation rate, plasma osteocalcin levels, and ex vivo osteoblast gene expression 8 weeks post-ovariectomy are all reversed to levels of sham-operated controls. We thus provide proof-of-concept for the use of vitamin C as a skeletal anabolic supplement in the therapy of human osteoporosis.