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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC IMPROVEMENT OF FRUIT CROPS THROUGH FUNCTIONAL GENOMICS AND BREEDING

Location: Appalachian Fruit Research Laboratory: Innovative Fruit Production, Improvement and Protection

Title: Advances in the genetic improvement of Prunus domestica utilizing genetic engineering

Authors
item SCORZA, RALPH
item CALLAHAN, ANN
item DARDICK, CHRISTOPHER
item SRINIVASAN, CHINNATHAMBI
item Raines, Charles
item Delong, Ted -
item Castro, Sarah -
item Abbott, Albert -
item Ravelonandro, Michel -
item Cambra, Mariano -
item Zagrai, Ioan -
item Polak, Jaroslav -
item Malinowski, Tadeusz -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: April 23, 2012
Publication Date: May 20, 2012
Citation: Scorza, R., Callahan, A.M., Dardick, C.D., Srinivasan, C., Raines, C.D., Delong, T., Castro, S., Abbott, A., Ravelonandro, M., Cambra, M., Zagrai, I., Polak, J., Malinowski, T. 2012. Advances in the genetic improvement of Prunus domestica utilizing genetic engineering. [abstract]. Xth International Symposium Plum & Prune Genetics. Paper No. 97.

Technical Abstract: Plum producers world-wide are facing multiple challenges including climate change, reductions in available labor, the need for reduced chemical inputs, the spread of native and exotic pests and pathogens, and consumer demands for improved fruit quality and health benefits. Meeting these challenges will require innovation in many areas of science and technology and especially in plum breeding. In an effort to develop new approaches to plum improvement, the USDA-ARS Appalachian Fruit Research Station fruit breeding program in collaboration with partners in the U.S. and Europe have developed a genetic engineering (GE) approach to target resistance to Plum pox virus (PPV) the causal agent of sharka, one of the most destructive diseases of plum. This program has resulted in the development of a GE plum variety ‘HoneySweet’ which has been tested for 15 years in the European Union and in the U.S., and is highly resistant to PPV. ‘HoneySweet’ has received full regulatory approval in the U.S. ‘HoneySweet’ represents a new source of PPV resistance for growers and it can be used by breeders to develop additional resistant varieties and/or rootstocks. To rapidly incorporate important traits into new plum varieties requires new approaches to breeding that can reduce or eliminate breeding limitations such as long juvenility periods; the need for extensive and costly breeding plots; and yearly limitations on flowering and fruiting related to seasonal dormancy. To address these limitations, the USDA group and partners in the U.S. have developed a system to shorten the breeding cycle of plum. We have overcome the juvenility and environmental limitations of flowering and fruiting by incorporating a gene that induces trees to flower early and continually. We have reduced the plum generation cycle from 3-7 years to less than 1 year. We call this rapid breeding system “FasTrack”. The system allows for the rapid incorporation of important traits into plums and then in the final generation, when substantial improvements are clearly evident, only seedlings that do not contain the early flowering transgene are selected. These trees are not genetically engineered. The selected trees may then be used directly as new varieties, or improved lines for further breeding. Genetic engineering of important traits, FasTrack breeding, and other approaches that are under development will allow the latest advances in biology to be applied to improving and sustaining plum production.

Last Modified: 8/19/2014
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