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United States Department of Agriculture

Agricultural Research Service

Research Project: INTEGRATED APPROACHES FOR PROTECTION OF ANIMALS FROM VECTOR-BORNE PATHOGENS

Location: Arthropod-Borne Animal Diseases Research

Title: The affect of Pseudomonas aeruginosa ingestion on antimicrobial peptide (AMP) and lysozyme expression in the common housefly (Musca domestica L.)

Authors
item Joyner, Chester -
item Mills, Mary -
item Evett, Christopher -
item Nayduch, Dana

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 30, 2011
Publication Date: July 30, 2011
Repository URL: http://esa.confex.com/esa/2011/webprogram/Paper58605.html
Citation: Joyner, C., Mills, M.K., Evett, C., Nayduch, D. 2011. The affect of Pseudomonas aeruginosa ingestion on antimicrobial peptide (AMP) and lysozyme expression in the common housefly (Musca domestica L.). Meeting Abstract. 58605.

Technical Abstract: The common house fly (Musca domestica L.) lives and thrives in one of the most septic niches on Earth, making bacterial exposure imminent. House flies often ingest bacteria and have both physical and chemical defenses utilized to protect themselves from harmful microbes. For example, defense molecules such as antimicrobial peptides (AMPs) and lysozyme are manufactured by tissues both locally (gut epithelium) and systemically (fat body). These molecules serve to neutralize or lyse bacteria, yet some species are still able to persist through the digestive tract, making the fly a potential biological vector. We found that Pseudomonas aeruginosa was able to survive passage through the fly alimentary canal and exit the digestive tract by 10 h post-ingestion. This study aimed to elucidate the systemic and local AMP and lysozyme responses in house flies after P. aeruginosaingestion. We analyzed the temporal expression of three AMPs (cecropin, defensin and diptericin) and lysozyme 1, 2, 6, 8, and 10 h after ingesting a known amount of P.aeruginosa. Tissue-specific expression was measured on both the mRNA and protein level using qRT-PCR and Western analysis. It is suspected that P. aeruginosa is able to pass through the fly alimentary canal either due to AMP and lysozyme resistance, or by bacteria altering the expression of AMPs and/or lysozyme in order to enhance their survival. By understanding the spatial and temporal molecular interactions between house fly defenses and P. aeruginosa, we can better understand the potential flies have for vectoring these bacteria.

Last Modified: 4/23/2014