Adenovirusmediated interference of FABP4 regulates ADIPOQ, LEP and LEPR expression in bovine adipocytes

Authors: WEI, S - Northwest Agriculture And Forestry University; ZAN, L - Northwest Agriculture And Forestry University; WANG, H - Northwest Agriculture And Forestry University; CHENG, G - Northwest Agriculture And Forestry University; DU, M - Washington State University; JIANG, Z - Washington State University; Hausman, Gary; DODSON, M - Washington State University

Submitted to: Genetics and Molecular Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/11/2012
Publication Date: N/A
Citation: N/A

Interpretive Summary: A fat cell protein that binds fatty acids plays an important role in regulating fatty acids in fat cells and it is is related to obesity, insulin resistance and intramuscular fat content. Varying the levels of this fatty acid binding protein do not influence the function of fat cells. But the levels of leptin were reduced when the level of the fatty acid binding protein was decreased. Leptin, which controls feeding level, may be controlled by altering the level of fatty acid levels in fat cells.

Technical Abstract: Fatty acid binding protein 4 plays an important role in fatty acid transportation in adipocytes and its expression is related to obesity, insulin resistance, metabolic syndrome and intramuscular fat content. Yet little is understood about FABP4 functions at the cellular level in the bovine. Thus, we constructed an adenovirus vector carrying small hairpin RNA against bovine FABP4 (AD-FABP4) and transfected bovine preadipocytes successfully. Results suggest that lower FABP4 did not alter levels of bovine-derived adipocyte differentiation, or differentiation-related gene expression at 24 h after AD-FABP4 treatment. However, knocking down (partially silencing) FABP4 decreased ADIPOQ and LEP expression, but increased LEPR levels, at 24 h and 72 h after AD-FABP4 treatment of bovine preadipocytes.