Submitted to: American Phytopathogical Society Southern Division
Publication Type: Abstract Only
Publication Acceptance Date: January 15, 2012
Publication Date: February 1, 2012
Citation: Feng, S., Chen, X., Holbrook Jr, C.C., Culbreath, A., Guo, B. 2012. Identification of putative TSWV resistance gene and development of gene-specific marker in peanut [abstract]. American Phytopathogical Society Southern Division. 102:52.3. Technical Abstract: Tomato spotted wilt virus (TSWV) is one of the most destructive viral diseases threatening peanut production in the Southeastern U.S. Among different strategies of controlling this disease, the use of resistant cultivars is more efficient. The objective of this study is to develop putative TSWV resistance gene-specific marker(s) based on peanut EST sequence homology to tomato Sw-5. One peanut EST sequence with approximately 37% amino acid similarity to tomato Sw-5 was further investigated. Two full length cDNA sequences were achieved using 3’- and 5’-RACE, named Ahsw-1 and Ahsw-2, respectively. Southern blot analysis reviewed that there were at least 4 copies of Ahsw genes in the allotetraploid peanut genome. RFLP polymorphisms have been observed among four parental lines of two RIL populations, suggesting PCR-based gene-specific markers could be developed. Three primer pairs have been designed and used to amplify the four parental lines for polymorphism and the RILs for mapping. One PCR-based maker from the Ahsw-2 gene appeared to fit 1:1 segregation ratio. Further study will conduct spatio-temporal expression patterns to confirm the possible relationship with the resistance to TSWV. More PCR primers will be used such as recent published Sw5 gene-based markers in tomato. These markers will be used in mapping population and QTL analysis.