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United States Department of Agriculture

Agricultural Research Service

Research Project: IMPROVING BIOCHEMICAL PROCESSES FOR THE PRODUCTION OF SUSTAINABLE FUELS AND CHEMICALS

Location: Renewable Product Technology Research Unit

Title: Novel antibacterial polypeptide produced by Lactobacillus paracasei strain NRRL B-50314

Authors
item Liu, Siqing
item Wilkinson, Brian -
item Bischoff, Kenneth
item Hughes, Stephen
item Rich, Joseph
item Cotta, Michael

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: June 19, 2012
Publication Date: June 19, 2012
Citation: Liu, S., Wilkinson, B.J., Bischoff, K.M., Hughes, S.R., Rich, J.O., Cotta, M.A. 2012. Novel antibacterial polypeptide produced by Lactobacillus paracasei strain NRRL B-50314 [abstract]. American Society for Microbiology. Paper #2512. p. 202.

Technical Abstract: This study reports the production and characterization of a novel antibacterial polypeptide, designated as laparaxin, which is secreted by Lactobacillus paracasei NRRL B-50314. The crude laparaxin has antibacterial activity against a range of Gram-positive bacteria including the following: lactic acid bacteria strains Lactococcus lactis LM 0230 and Lactobacillus buchneri NRRL B-30929 (isolated from a bio-ethanol production facility); the food-borne pathogen Listeria monocytogenes; the gastrointestinal pathogen Enterococcus faecalis; strains of the opportunistic pathogen Staphylococcus aureus including methicillin-resistant S. aureus (MRSA) 592S, 209P, 1316V5, 1316V20, DU496S, and a hetero-vancomycin-intermediate methicillin resistant strain (HVI-MRSA) MM66. Using L. lactis as an indicator strain, the inhibitory activity of crude laparaxin was detected initially in early log phase, and the activity maximizes at the early stationary phase and remains stable after prolonged incubation. Laparaxin activity is stable when incubated at 94**oC for 30 min. Higher concentrations of inhibitory activity are produced when glucose, fructose, and sucrose were used as carbon-sources in growth media. The crude laparaxin has potential applications in food and feed industry as well as in clinical and veterinary medicine.

Last Modified: 4/20/2014
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