Title: Microbial screening and analytical methods for the production of polyol oils from soybean oil Authors
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 2, 2012
Publication Date: May 2, 2012
Citation: Hou, C.T., Ray, K.J. 2012. Microbial screening and analytical methods for the production of polyol oils from soybean oil [abstract]. American Oil Chemists' Society. Paper #BIO-3. Technical Abstract: The objective of this study is to develop a new useful method including microbial screening and product identification for a bioprocess to produce polyol oils from soybean oil. Methods for separating of product polyol oils from soybean oil substrate and free fatty acid byproducts using HPLC and TLC were established. With HPLC, a C18 reverse phase column and a gradient of 100% methanol to 100% 2-propanol over 60 minutes produced the best result. Free fatty acids were eluted at between 4.5 min to 11 min. Polyol oil products were eluted at between 15 min to 26 min. The substrate soybean oil was eluted at between 35 min to 44 min. A TLC method with two step development solvent system was also developed to separate polyol oil products from substrate. A total of 100 microbial cultures were screened and found 25 hits. Among these 25 hits, Pseudomonas sp. A01-35 was the best strain for the production of polyol oil from soybean oil. Polyol oil products were purified and their chemical structures confirmed by HPLC/MS. This is a useful microbial screening and analytical method for the production of polyols oils directly from soybean oil.