GENETIC IMPROVEMENT OF BARLEY
Location: Cereal Crops Research
Title: Testing transgenic spring wheat and barley lines for reaction to Fusarium head blight: 2011 field nursery report.
| Syverson, R - |
| Elakkad, A - |
| Shah, J - |
| Dill-Macky, Ruth - |
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 5, 2011
Publication Date: December 4, 2011
Citation: Syverson, R.L., A.M. Elakkad, L.S. Dahleen, V.J. Nalam, G. Klossner, J. Shah, and R. Dill-Macky. 2011. Testing transgenic wheat and barley lines for reaction to Fusarium head blight: 2011 field nursery report. In: S. Canty, A. Clark, A. Anderson-Scully, D. Ellis, and D. Van Sanford (eds), Proceedings of the 2011 National Fusarium Head Blight Forum (p. 97). East Lansing, MI/Lexington, KY: U.S. Wheat & Barley Scab Initiative.
The 2011 field screening nursery, with 56 wheat and 88 barley plots was located at UMore Park, Rosemount MN. Trial entries and untransformed controls were submitted by the University of North Texas (9+1 wheat), and USDA (17+2 barley). Lines with known reactions to Fusarium head blight (FHB) were also included as checks. The wheat checks used were the moderately resistant Alsen, the susceptible cultivars Wheaton and Roblin, and a non-inoculated Wheaton check. The barley checks were the moderately resistant Quest and the susceptible cultivars Conlon (2-rowed), Robust and Stander. The experimental design was a randomized block with four replicates. Plots were 2.4 m long single rows. The trial was planted on May 18, 2011. All plots, except a non-inoculated Wheaton check, were inoculated twice. The first inoculation was applied at anthesis for wheat and at head emergence for barley. The second inoculation was applied three days after the initial inoculation (dai) for each plot. The inoculum was a composite of 50 F. graminearum isolates at a concentration of 100,000 (barley) or 200,000 (wheat) macroconidia.ml-1 with Tween 20 (polysorbate) added at 2.5 ml.L-1 as a wetting agent. The inoculum was applied using a CO2-powered backpack sprayer fitted with a SS8003 TeeJet spray nozzle with an output of 10ml.sec-1 at a working pressure of 275 kPa. Mist-irrigation was applied from the first inoculation on July 7 till July 25 to facilitate FHB development. FHB incidence and severity were assessed visually 17 d.a.i. for wheat and 13 d.a.i. for barley on 20 arbitrarily selected heads per plot. FHB incidence was determined by the percentage of spikes with visually symptomatic spikelets of the 20 heads observed. FHB severity was determined as the percentage symptomatic spikelets of the total of all spikelets observed. Plots were harvested at maturity on August 5 (barley) and 11 (wheat). Fifty (barley) and 30 (wheat) heads where harvested from each plot, and were threshed and the seed cleaned by hand. The wheat sub-samples were used to determine the percentage of Fusarium damaged kernels (FDK) and then all samples (wheat and barley) were ground and submitted for deoxynivalenol (DON) analysis. The data indicated that resistance was expressed in some of the transformed lines.