ARS | Publication request: Increased adiposity induced by high dietary butter oil increases vertebrae trabecular structural indices in rats

Submitted to: Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: November 13, 2011
Publication Date: March 29, 2012
Citation: Nielsen, F.H. 2012. Increased adiposity induced by high dietary butter oil increases vertebrae trabecular structural indices in rats. Experimental Biology. 26:265.1.

Technical Abstract: Obesity has been associated with both improved and impaired bone health, and other dietary factors apparently affect the nature of the association. An experiment was performed to determine whether increased adiposity induced by high dietary butter oil impairs bone structure and whether that effect is exacerbated by moderate magnesium (Mg) deficiency. Groups of 24-25 male Sprague-Dawley rats aged 6 wk were fed dietary Mg at 250, 500, or 750 mg/kg and dietary fat at 10% (4.4% butter oil, 5.6% soybean oil) or 45% (39.6% butter oil, 5.4% soybean oil) of total energy. After 24 wk, 12 rats from each group fed high dietary butter oil exhibited increased % and g fat (determined by MRI) and g epididymal fat. Decreased plasma 1, 25-(OH)2-vitamin D and parathyroid hormone and bone magnesium indicated the rats fed the low-Mg/diet were Mg-deficient. Vertebrae trabecular amount, number, thickness, and connectivity were increased by the high butter oil feeding. Moderate Mg deficiency did not affect vertebrae micro architecture. Moderate Mg deficiency decreased femur strength (force to break), which effect, was alleviated by high dietary butter oil. Findings suggest that a rat with increased adiposity caused by high dietary butter oil is not a suitable model for the determination of whether Mg deficiency modifies impaired bone health associated with obesity.