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United States Department of Agriculture

Agricultural Research Service

Research Project: ALLIUM, CUCUMIS, AND DAUCUS GERMPLASM ENHANCEMENT, GENETICS, AND BIOCHEMISTRY

Location: Vegetable Crops Research Unit

Title: Transcriptome profilng of defense responses to aphid feeding in wheat

Authors
item Krishnareddy, Srirama -
item Weng, Yiqun
item Rudd, Jackie -
item Akhunova, Alina -
item Liu, Shuyu -

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: November 20, 2011
Publication Date: December 15, 2011
Citation: Krishnareddy, S., Weng, Y., Rudd, J., Akhunova, A., Liu, S. 2011. Transcriptome profilng of defense responses to aphid feeding in wheat [abstract]. Plant and Animal Genome Conference. Available: http://pag.confex.com/pag/xx/webprogram/Paper4178.html.

Technical Abstract: Greenbug (Schizaphis graminum) is a serious aphid pest in small grain crops in the southern Great Plains of the US. We are trying to understand the molecular mechanisms of host resistance against aphid infestation in the grass genome using wheat-greenbug as a model system. In the present study, a microarray experiment was conducted to investigate transcriptomic defense responses in the resistant (TXGBE273) and susceptible (TXGBE281) near isogenic wheat lines of the greenbug resistance gene Gb3 using Affymetrix wheat GeneChips. At 0, 24, and 48 hours after biotype E infestation, bulked resistant and susceptible pools of leaf samples from F7 recombinant inbred lines of TXGBE273 × TXGBE281 NILs were prepared and used for total RNA extraction with three biological replications. Analysis of microarray data suggested that, within 24 hours after greenbug feeding, significant changes were observed between resistant and susceptible bulks in the expression of genes related to a number of bioligcal processes including plant hormone and amino acid biosynthesis, metabolism, cell wall modification, defense signaling, secondary metabolites. These results provided an excellent opportunity to understand the molecular basis of Gb3-mediated resistance mechanisms to greenbug feeding.

Last Modified: 9/23/2014