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United States Department of Agriculture

Agricultural Research Service

Research Project: SUBTROPICAL INSECT PESTS OF VEGETABLES AND ORNAMENTAL PLANTS

Location: Subtropical Insects and Horticulture Research

Title: Isothermal amplification of insect DNA

Authors
item Dickey, Aaron
item Osborne, Lance -
item Shatters, Robert
item McKenzie, Cindy

Submitted to: Entomological Society of America Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: September 8, 2011
Publication Date: November 16, 2011
Citation: Dickey, A.M., Osborne, L.S., Shatters, R.G., Mckenzie, C.L. 2011. Isothermal amplification of insect DNA [abstract]. Entomological Society of America Annual Meeting. Paper No. D0542.

Technical Abstract: The loop-mediated isothermal amplification of DNA (LAMP) method can amplify a target DNA sequence at a constant temperature in about 1 hour. LAMP has broad application in agriculture and medicine because of the need for rapid and inexpensive diagnoses. The power of LAMP is being used by researchers and clinicians for pathogen detection and positive identification but agricultural applications have been much more limited. LAMP should be well suited to the identification of economically and medically important insects, particularly for members of cryptic species complexes, which cannot be distinguished morphologically. Here we report the first loop-mediated isothermal amplification of insect DNA using sweetpotato whitefly Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae) biotypes B and Q.

Last Modified: 9/2/2014