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United States Department of Agriculture

Agricultural Research Service

Research Project: Integrated Aquatic Animal Health Strategies

Location: Aquatic Animal Health Research

Title: Evaluation of an in vitro cell assay to select attenuated bacterial mutants of Aeromonas hydrophila and Edwardsiella tarda to channel catfish

Authors
item Wei Pridgeon, Yuping
item Klesius, Phillip
item Mu, Xingjiang -

Submitted to: Journal of Applied Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: August 29, 2011
Publication Date: January 9, 2012
Repository URL: http://hdl.handle.net/10113/55453
Citation: Wei Pridgeon, Y., Klesius, P.H., Mu, X. 2012. Evaluation of an in vitro cell assay to select attenuated bacterial mutants of Aeromonas hydrophila and Edwardsiella tarda to channel catfish. Journal of Applied Microbiology. 111:1310-1318.

Interpretive Summary: The possibility of using fish cells instead of live fish to select attenuated bacterial mutants was evaluated in this study. Correlation analysis between the two assays revealed that there was a significant correlation between the two assays, indicating that the fish cell assay could be initially used to screen large quantities of bacteria to select attenuated mutants for the purpose of novel vaccine development. The use of this fish cell assay will reduce the cost involved in the live fish assays which require many fish and aquariums.

Technical Abstract: To evaluate the feasibility of using an in vitro cell assay to select attenuated bacterial mutants. Using catfish gill cells G1B, the feasibility of using an in vitro assay instead of in vivo virulence assay using live fish to select attenuated bacterial mutants was evaluated in this study. Pearson correlation analysis between in vitro virulence to G1B cells and in vivo virulence of Aeromonas hydrophila and Edwardsiella tarda revealed that there was a significant correlation between the two (r = - 0.768, P value = 3.7 x 10 -16). The in vitro cell assay might be initially used to screen large quantities of bacteria to select attenuated mutants of catfish pathogens. The in vitro cell assay using catfish gill cells to identify attenuated mutants of catfish pathogens will reduce cost involved in the in vivo virulence assay which requires many fish and aquariums.

Last Modified: 10/20/2014
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