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United States Department of Agriculture

Agricultural Research Service

Research Project: IMPROVING BIOCHEMICAL PROCESSES FOR THE PRODUCTION OF SUSTAINABLE FUELS AND CHEMICALS

Location: Renewable Product Technology Research Unit

Title: Vntr Fingerprinting of Kluyveromyces Marxianus Strains Wt, 7-1, and 8-1 by Using Different Primer Types to Give Best Results in Pcr and on Electrophorese Gel in Order to Find Differentiation of the DNA of the Yeast Strains.

Authors
item Schoepke, Andrew -
item Cox, Elby -
item Rich, Joseph
item Hughes, Stephen

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: October 13, 2011
Publication Date: October 13, 2011
Citation: Schoepke, A., Cox, E., Rich, J.O., Hughes, S.R. 2011. VNTR fingerprinting of Kluyveromyces marxianus strains WT, 7-1, and 8-1 by using different primer types to give best results in PCR and on electrophorese gel in order to find differentiation of the DNA of the yeast strains [abstract]. American Chemical Society. Poster #11.

Technical Abstract: Using mutagenized Kluyveromyces marxianus strains (WT, 7-1, 8-1) we wish to find out the variable numbered tandem repeats (VNTR) of each of the DNA strains from the different mutagenized K. marxianus strains. To do this we used Phusion HF Buffer Pack to try and give a clear picture of the VNTR by using different fungal primers to try and figure out the genetic fingerprint of each yeast strain. If we can find the differences in the VNTR this may be able to tell us as to why which yeast grows best in glucose then the others and can produce more ethanol.

Last Modified: 7/30/2014
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