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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC AND CULTURAL PRACTICE IMPROVEMENT FOR SUSTAINABLE COTTON PRODUCTION Title: Mapping and validation of fiber strength quantitative trait loci on chromosome 24 in Upland cotton

Authors
item Kumar, Pawan -
item Singh, Rippy -
item Lubbers, Edward -
item Shen, Xinlian -
item Paterson, Andrew -
item Campbell, Benjamin
item Jones, Donald -
item Chee, Peng -

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 3, 2012
Publication Date: May 1, 2012
Citation: Kumar, P., Singh, R., Lubbers, E.L., Shen, X., Paterson, A.H., Campbell, B.T., Jones, D.C., Chee, P.W. 2012. Mapping and validation of fiber strength quantitative trait loci on chromosome 24 in Upland cotton. Crop Science. 52:1115-1122.

Interpretive Summary: A major fiber strength QTL (putative gene) has been identified on chromosome 24 in the Chinese germplasm line “Suyuan 7235.” In this study, we confirmed the effects of this QTL in different genetic backgrounds by crossing Suyuan 7235 with two U.S. germplasm lines with different fiber strength. Results showed that the QTL was consistently expressed in both populations. The beneficial Suyuan 7235 allele explained up to 40% of the total phenotypic variation and accounted for an increase of up to 11.8 kN m/kg. The effects on fiber strength appear to be greater in Pop-883 than in Pop-542 despite the SL-883 parent having stronger fiber than the SL-542 parent. DNA fingerprinting on a collection of elite cotton lines indicated that this QTL is not present in U.S. public germplasm. These results indicate that this fiber strength QTL could significantly improve the economic value of Upland cottons in the U.S.

Technical Abstract: A major fiber strength QTL has been identified on chromosome 24 in the Chinese germplasm line “Suyuan 7235,” however the effects of this QTL have not been tested in different genetic backgrounds. In this study, we confirmed the effects of this QTL by crossing Suyuan 7235 with two U.S. germplasm lines with different fiber strength. This QTL was consistently expressed in both populations, and over generations and year. The Suyuan 7235 allele explained up to 40% of the total phenotypic variation and accounted for an increase of up to 11.8 kN m/kg. The effects on fiber strength appear to be greater in Pop-883 than in Pop-542 despite the SL-883 parent having stronger fiber than the SL-542 parent. DNA fingerprinting on a collection of elite cotton lines indicated that this QTL is not present in U.S. public germplasm. These results indicate that this fiber strength QTL could significantly improve the economic value of Upland cottons in the U.S. The identification of 27 novel markers tightly-linked in this region adds an additional toolkit to allow this QTL to be more efficiently deployed in breeding cultivars with improved fiber strength.

Last Modified: 10/25/2014