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United States Department of Agriculture

Agricultural Research Service

Research Project: IMPROVING GRAPE ROOTSTOCK AND SCION PEST AND DISEASE RESISTANCE Title: Characterization of polyphenolic metabolites in the seeds of Vitis germplasm

Authors
item Liang, Zhenchang -
item Yang, Yingzhen
item Cheng, Lailiang -
item Zhong, Gan-Yuan

Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: October 10, 2011
Publication Date: January 26, 2012
Citation: Liang, Z., Yang, Y., Cheng, L., Zhong, G. 2012. Characterization of polyphenolic metabolites in the seeds of Vitis germplasm. Journal of Agricultural and Food Chemistry. 60(5):1291-1299.

Interpretive Summary: Grapes are rich in many compounds that have significant benefits to human nutrition and health. Polyphenols are one class of such compounds. In this study, we evaluated the composition and content of polyphenols in the seed of 91 grape accessions from 17 grape species in 2 consecutive years. A total of 28 polyphenolic compounds were detected and quantified. Several of the polyphenolic compounds detected in this study, such as flavonols, are known to be important to human health. Among the 17 species investigated, V. palmata, V. vinifera and V. vulpina were found to have significantly higher levels of total polyphenolic content than the other species. A number of accessions possessing high content of various individual groups of seed polyphenolic compounds were also identified. Many accessions used in this study are maintained in the USDA-Agricultural Research Service, Plant Genetic Resources Unit in Geneva, New York. This study will be an important addition to our ongoing effort in developing a comprehensive database of nutrition- and health-related secondary metabolites in the grape germplasm for grape improvement.

Technical Abstract: The composition and content of polyphenolic compounds in the seeds of 91 grape accessions from 17 Vitis species were characterized for two consecutive years. A total of 28 polyphenolic compounds including 2 gallic derivatives, 3 monomer flava-3-ols, 15 dimer flava-3-ols, 4 trimer flava-3-ols, resveratrol and 3 flavonols, were identified via HPLC-MS and quantified by HPLC-DAD. Tremendous variation in these six groups of compounds was observed both among and within the species studied. Monomer flava-3-ols were the most abundant, followed by dimerc and trimer flava-3-ols. They collectively accounted for more than 96% of the total variation. V. palmata, V. vinifera and V. vulpina were found to have significantly higher levels of total polyphenolic content than other species. A number of accessions possessing high content of various individual groups of seed polyphenolic compounds were also identified. This study will be an important addition to our ongoing effort in developing a comprehensive database of nutrition- and health-related secondary metabolites in the Vitis germplasm for grape improvement.

Last Modified: 12/22/2014
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