|Moraes, Rita -|
|Lata, Hemant -|
|Sumyanto, Joko -|
|Pereira, Ana -|
|Bertoni, Bianca -|
|Joshi, Vaishali -|
|Pugh, Nirmal -|
|Khan, Ikhlas -|
|Pasco, David -|
Submitted to: Plant Cell Tissue And Organ Culture
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 17, 2011
Publication Date: February 1, 2011
Citation: Moraes, R.M., Lata, H., Sumyanto, J., Pereira, A.M., Bertoni, B.W., Joshi, V.C., Pugh, N.D., Khan, I.A., Pasco, D.S. 2011. Characterization and pharmacological properties of in vitro propagated clones of Echinacea tennesseensis (Beadle) small. Plant Cell Tissue And Organ Culture. 106:309-315. Interpretive Summary: The leaves and roots of Echinacea tennesseensis, a coneflower found only in the state of Tennessee, has immune enhancing properties. Our results show that new plants, whether produced by seed or tissue culture and regardless of differences in leaf shape length and size, still exhibit similar medicinal properties.
Technical Abstract: Tissue culture techniques have been used to establish and maintain a repository of medicinal Echinacea. In vitro clones obtained from hypocotyls of germinated seeds, varied macroscopically, microscopically and exhibited variation in immune enhancing activity. Two in vitro produced clones of Echinacea tennesseensis (Beadle) Small (ETN 03 and ETN 11) were identified as high and low activity based on the activation of human monocytes. Phenotypic analyses of ETN 03 and ETN 11 clones were done using AFLP (Amplified Fragment Length Polymorphism) assay. Results of the AFLP assay revealed that no mutation has occurred during in vitro multiplication, storage, and acclimatization into soil. Plants of ETN 03, ETN 11 clones were cultivated for two growing seasons. Extracts of their dry leaves and roots exhibited immune enhancing activity; however, the variation in activity noticed between clones during micropropagation diminished and was no longer statistically relevant.