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ARS Home » Southeast Area » Raleigh, North Carolina » Soybean and Nitrogen Fixation Research » Research » Publications at this Location » Publication #269527
Title: Glycine max cultivar Dare chloroplast fatty acid desaturase 7 (FAD7) gene, complete cds; nuclear gene for chloroplast product

Author
item Upchurch, Robert
item Ramirez, Martha

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Research Notes
Publication Acceptance Date: 11/10/2010
Publication Date: 5/10/2011
Citation: Upchurch, R.G., Ramirez, M.E. 2011. Glycine max cultivar Dare chloroplast fatty acid desaturase 7 (FAD7) gene, complete cds; nuclear gene for chloroplast product. National Center for Biotechnology Information (NCBI). Published online.

Interpretive Summary: Soybean fatty acid desaturase 7 (GmFAD7) is a nuclear encoded gene whose protein product is transported into the chloroplast membranes. We sequenced the genomic FAD7 gene in order to characterize gene structure and to determine FAD7 gene expression in response to cold stress and pathogen infection of soybean leaves. The genomic sequence of soybean GmFAD7 (2455 nucleotides) was determined and reported to GenBank and was given the accession number HM769340. The structure and deduced amino acid sequence of soybean FAD7 is similar to other higher plant plastidal omega-3 desaturases: 8 exons and 7 introns, predicted proteins of 453 amino acid residues containing three conserved histidine box motifs, amino terminal chloroplast transit peptides, and a molecular mass of 51.3. GmFAD7 has 76% amino acid sequence identity to GmFAD8.

Technical Abstract: The genomic sequence of soybean GmFAD7 (2455 nucleotides) was determined and reported to GenBank and assigned the accession number HM769340. The structure and deduced amino acid sequence of soybean FAD7 is similar to other higher plant plastidal omega-3 desaturases: 8 exons and 7 introns, predicted proteins of 453 amino acid residues containing three conserved histidine box motifs, amino terminal chloroplast transit peptides, and a molecular mass of 51.3. GmFAD7 has 76% amino acid sequence identity to GmFAD8. GmFAD7 exons range in size from 67 to 521 nucleotides and the 7 introns range in size from 90 to 393 nucleotides. Two complete copies of GmFAD7, one on chromosome 18 and one on chromosome 7 of the Williams 82 soybean genome were found with strong sequence similarity to GmFAD7 of cultivar Dare. Comparison of the Dare GmFAD7 peptide sequence to published soybean FAD7 peptides revealed 98% identity to the homologous sequences.