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United States Department of Agriculture

Agricultural Research Service

Research Project: DISCOVERY AND UTILIZATION OF BIOACTIVE COMPONENTS FROM NEW CROPS AND AGRICULTURAL CO-PRODUCTS

Location: Functional Foods Research Unit

Title: Optimized analysis and quantification of glucosinolates from Camelina sativa seeds by reverse-phase liquid chromatography

Authors
item Berhow, Mark
item Polat, Umit -
item Glinski, Jan -
item Glensk, Michal -
item Vaughn, Steven
item Isbell, Terry
item Ayala-Diaz, Ivan -
item Marek, Laura -
item Gardner, Candice

Submitted to: Industrial Crops and Products
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 11, 2012
Publication Date: July 18, 2012
Citation: Berhow, M.A., Polat, U., Glinski, J.A., Glensk, M., Vaughn, S.F., Isbell, T., Ayala-Diaz, I., Marek, L., Gardner, C.A. 2013. Optimized analysis and quantification of glucosinolates from Camelina sativa seeds by reverse-phase liquid chromatography. Industrial Crops and Products. 43:119-125.

Interpretive Summary: Gold-of-pleasure or false flax (Camelina sativa L. Crantz) presscake contains three relatively unique glucosinolates that have not been assessed for their phytochemical activities in agriculture or their nutritional/pharmacological roles in animals due to the lack of availability of purified standards for study. Using defatted seed material, we have developed methods to isolate milligram quantities of these glucosinolates using a combination of liquid chromatographic methods. Preliminary experiments with centrifugal partition chromatography produced encouraging results suggesting this technique could be suitable for purification of gram quantities of these glucosinolates. Seeds from 30 cultivars grown in a U.S. field trial were evaluated and shown to have total glucosinolate concentrations that ranged from 7.7 to 14 mg/g in the defatted meal. Sprouted camelina seeds were shown to have decreasing levels of glucosinolates over seven days of sprouting. This study will be used to develop new uses for camelina seed and seedmeal based on glucosinolate content.

Technical Abstract: Gold-of-pleasure or false flax (Camelina sativa L. Crantz) presscake contains three relatively unique glucosinolates: glucoarabin (9-(methylsulfinyl) nonylglucosinolate)glucocamelinin (10-(methylsulfinyl)decylglucosinolate), and 11-(methylsulfinyl)undecylglucosinolate. Using defatted seed material, we have developed methods to isolate mg quantities of these glucosinolates using a combination of reverse phase flash and preparative HPLC methods. Preliminary experiments with centrifugal partition chromatography produced encouraging results suggesting this technique could be suitable for purification of gram quantities of these glucosinolates. Seeds from 30 cultivars grown in a U.S. field trial were evaluated and shown to have total glucosinolate concentrations that ranged from 7.7 to 14 mg/g in the defatted meal. Sprouted camelina seeds were shown to have decreasing levels of glucosinolates over seven days of sprouting. These glucosinolates have not been assessed for their phytochemical activities in agriculture or their nutritional/pharmacological roles in animals due to the lack of availability of purified standards for study. This study will be used to develop new uses for camelina seed and seedmeal based on glucosinolate content.

Last Modified: 9/1/2014
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