Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: August 18, 2011
Publication Date: November 14, 2012
Citation: Hall, M. 2012. The glucose oxidase-peroxidase assay for glucose. In: Preedy, V.R., editor. Dietary Sugars: Chemistry Analysis, Function and Effects. Cambridge, United Kingdom: RSC Publishing. Chapter 20, p. 340-357.
The glucose oxidase-peroxidase assay for glucose has served as a very specific, sensitive, and repeatable assay for detection of glucose in biological samples. It has been used successfully for analysis of glucose in samples from blood and urine, to analysis of glucose released from starch or glycogen in human foods, animal feeds, and diverse plant, animal, and microbial samples. The chemistry, methodology, performance, and considerations in successful application of this assay are discussed.