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United States Department of Agriculture

Agricultural Research Service

Research Project: IMPROVING BIOCHEMICAL PROCESSES FOR THE PRODUCTION OF SUSTAINABLE FUELS AND CHEMICALS

Location: Renewable Product Technology Research Unit

Title: Why engineering lactic acid bacteria for biobutanol

Author
item Liu, Siqing

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 28, 2011
Publication Date: July 28, 2011
Citation: Liu, S. 2011. Why engineering lactic acid bacteria for biobutanol [abstract]? Society for Industrial Microbiology. p. 75.

Technical Abstract: The Gram-positive Lactic acid bacteria (LAB) are considered attractive biocatalysts for biomass to biofuels for several reasons. They have GRAS (Generally Recognized As Safe) status that are acceptable in food, feed, and medical applications. LAB are fermentative: selected strains are capable of fermenting multiple sugars simultaneously and converting diversified substrates into various products; LAB are adaptive: capable of growth in lower or higher pHs, at a temperature range from 30oC-50oC. Most importantly, LAB can tolerate and grow in media supplemented with up to 3-4% butanol. Butanol has been recognized as a superior transportation fuel since it is more energy dense than ethanol and it can be used directly as a drop-in fuel without engine modification. Biologically, butanol is produced through anaerobic fermentation by Clostridia cultures. However, the overall economics for commercial butanol fermentation remains uncertain. Major hurdles include low fermentation efficiency with cheaper lignocellulosic feedstocks and high toxicity of butanol to its producing strains. The increased butanol concentrations (less than 2%) in a butanol fermentation bioreactor, albeit desired for cost-effective product recovery, often lead to reduced growth and eventually death of the producing Clostridium cells. Suitable microbial hosts to be used for metabolic engineering strategies for butanol production must be able to tolerate higher titers of butanol in order to improve yields. The butanol tolerant LAB strains need to be tested as potential hosts for manipulations toward efficient conversion of biomass to butanol. Results of using genetic engineering tools to modify selected LAB strains for butanol production will be presented.

Last Modified: 10/24/2014
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