NOVEL TECHNOLOGIES FOR PRODUCING RENEWABLE CHEMICALS AND POLYMERS FROM CARBOHYDRATES DERIVED FROM AGRICULTURAL FEEDSTOCKS
Location: Renewable Product Technology Research Unit
Title: Chemical characterization of carbohydrate-based biosurfactants
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: June 25, 2011
Publication Date: June 25, 2011
Citation: Price, N.P., Leathers, T.D., Kurtzman, C.P., Vermillion, K., Rooney, A.P., Manitchotpisit, P., Punnapayak, H. 2011. Chemical characterization of carbohydrate-based biosurfactants [abstract]. Gordon Conference on Carbohydrates. Poster #64.
High-yield, glycolipid-based biosurfactants are of increasing interest for use in environmentally benign cleaning or emulsifying agents. We have developed a MALDI-TOF/MS screen for the rapid analysis of several types of biosurfactants, including various acylated rhamnolipids in Pseudomonas extracts, and a newly identified sophorolipid-producing yeast. The approach is validated by carbohydrate and lipid GC/MS analysis, RP-HPLC, and 1D- and 2D-NMR. Unlike those reported previously, the Candida spp. NRRL Y-27208 sophorolipids contain a omega-hydroxy-linked acyl group (typically 18-hydroxy-delta-9-octadecenoate), and occur predominantly in a non-lactone, anionic form. In addition, seventeen dimeric and trimeric sophoroses were identified by MALDI-TOF/MS from this strain. We have also surveyed more than 50 diverse strains of Aureobasidium pullulans, representing at least 6 phylogenic clades, more than 40% of which produce unusual, denser-that-water biosurfactants. The complete structure of these A. pullulans biosurfactant has been elucidated, and is reported here the first time. These structures are novel, although with some characteristics of a related polyhydroxylated natural product called exophilin. The relatively high yield and surfactant-like properties of these new materials has potential value as replacements for petroleum-based detergents and emulsifiers.