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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC IMPROVEMENT OF SUGARCANE BY CONVENTIONAL AND MOLECULAR APPROACHES Title: A Lcp 85-384 Genetic Linkage Map Enriched with Polymorphic Ssr Markers

Authors
item Liu, Pingwu -
item Que, Youxiong -
item Andru, S -
item Kimbeng, Collins -
item Pan, Yong-Bao

Submitted to: American Society of Sugar Cane Technologists
Publication Type: Abstract Only
Publication Acceptance Date: May 5, 2011
Publication Date: June 8, 2011
Citation: Liu, P., Que, Y., Andru, S., Kimbeng, C.A., Pan, Y.-B. 2011. A LCP 85-384 genetic linkage map enriched with polymorphic SSR markers. Journal of the American Society of Sugar Cane Technologists. 31:57-58. Available: http://assct.org/journal/JASSCT%20PDF%20Files/Volume%2031/rpvASSCTabstracts2011.pdf

Technical Abstract: Sugarcane (Saccharum spp. hybrids) cultivars, such as Q165, R570 and LCP 85-384, have been used to construct genetic segregation populations for the development of genetic linkage maps. Based on the genetic linkage map for a selfed-progeny population of R570, the French research group at CIRAD tagged a rust resistance gene that eventually led to map-based cloning of the ‘Bru1’ gene. Louisiana sugarcane researchers at the USDA and the LSU AgCenter have taken a similar approach and constructed a preliminary genetic linkage map based upon a selfed-progeny population of LCP 85-384. The objective of this study was to enrich this linkage map with newly discovered polymorphic SSR markers. This enrichment process involved a total of 1, 504 polymorphic markers with 64 AFLP, 12 TRAP, and 83 SSR primer pairs. Nine hundred and ninety-three single dose markers (Theoretical segregation ratio <= 6.7:1) were chosen for map construction. Of which 634, including 465 AFLP, 70 TRAP, and 99 SSR markers, fit the theoretical single dose markers segregation ratio of 3:1. The remaining 359 distorted single dose markers did not fit the 3:1 segregation ratio. Eight hundred and eighty-four out of 993 single dose markers were successfully assigned onto 108 co-segregation groups (CGs). These 884 markers included 615 AFLP (69.6%), 90 TRAP (10.2%), and 179 SSR (20.2%) markers. An additional eight AFLP, one TRAP, and 163 SSR markers were added to the 108 CGs when comparing with the preliminary genetic linkage map. Sixty-three of the 108 CGs were successfully assigned into 11 homologous groups (HGs) based on the SSR marker information. The number of CGs for each HGs ranged from as few as two to as many as 27. This enriched LCP 85-384 genetic linkage map will be useful for mapping QTLs associated with important agronomic traits in sugarcane, such as sugar and fiber content.

Last Modified: 11/1/2014