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ARS Home » Southeast Area » Auburn, Alabama » Aquatic Animal Health Research » Research » Publications at this Location » Publication #265672
Title: Identification and expression profile of multiple genes in Nile tilapia in response to formalin killed Streptococcus iniae vaccination

Author
item Wei Pridgeon, Yuping
item Klesius, Phillip

Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/6/2011
Publication Date: 7/19/2011
Citation: Wei Pridgeon, Y., Klesius, P.H. 2011. Identification and expression profile of multiple genes in Nile tilapia in response to formalin killed Streptococcus iniae vaccination. Veterinary Immunology and Immunopathology. 142:201-206.

Interpretive Summary: Twenty-eight expressed sequence tags (ESTs) were isolated from a Nile tilapia vaccinated vs non-vaccinated subtractive library at 12- h post injection of a formalin killed S. iniae ARS-98-60 vaccine. The 28 ESTs were classified in terms of their putative functions. Half of the ESTs identified are unknown proteins. Of the rest half ESTs, 17% has putative functions in protein biosynthesis and 11% has putative functions in immunity, energy production, and signal transduction, respectively. Immunity-related ESTs identified included high density lipoprotein-binding protein vigilin, immunoglobulin heavy chain, and QM-like protein. Quantitative PCR revealed that one EST (cytochrome c oxidase subunit II) was upregulated 1825 fold in vaccinated fish compared to that in non-vaccinated fish. Of the rest 27 ESTs, nine were significantly upregulated (< 20 fold) in vaccinated fish. The nine significantly upregulated genes included five unknown or hypothetical proteins and four known proteins: high density lipoprotein-binding protein vigilin, QM-like protein, ribosomal protein S13, and ribosomal protein L5.

Technical Abstract: Twenty-eight expressed sequence tags (ESTs) were isolated from a Nile tilapia vaccinated vs non-vaccinated subtractive library at 12- h post injection of a formalin killed S. iniae ARS-98-60 vaccine. The 28 ESTs were classified in terms of their putative functions. Half of the ESTs identified are unknown proteins. Of the rest half ESTs, 17% has putative functions in protein biosynthesis and 11% has putative functions in immunity, energy production, and signal transduction, respectively. Immunity-related ESTs identified included high density lipoprotein-binding protein vigilin, immunoglobulin heavy chain, and QM-like protein. Quantitative PCR revealed that one EST (cytochrome c oxidase subunit II) was highly upregulated (1825 +/- 336 fold) in vaccinated fish compared to that in non-vaccinated fish. Of the rest 27 ESTs, nine were significantly (P < 0.05) upregulated (< 20 fold) in vaccinated fish. The nine significantly upregulated genes included five unknown or hypothetical proteins and four known proteins (high density lipoprotein-binding protein vigilin, QM-like protein, ribosomal protein S13, and ribosomal protein L5).