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United States Department of Agriculture

Agricultural Research Service

Research Project: IMPROVING ALFALFA AND OTHER FORAGE CROPS FOR BIOENERGY, LIVESTOCK PRODUCTION, AND ENVIRONMENTAL PROTECTION Title: Rna-Seq Atlas of White Lupin: a Guide to the Phosphorus Deficiency Response Pathway in Plants

Authors
item Yang, Suk
item Miller, Susan
item Liu, Junqi -
item Tu, Jin -
item Gronwald, John
item Vance, Carroll

Submitted to: American Society of Plant Biologists Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: August 6, 2011
Publication Date: August 6, 2011
Citation: Yang, S.H., Miller, S.S., Liu, J., Tu, J., Gronwald, J.W., Vance, C.P. 2011. RNA-Seq atlas of white lupin: a guide to the phosphorus deficiency response pathway in plants [abstract]. Plant Biology 2011, American Society of Plant Biologists Annual Meeting, August 6-10, 2011, Minneapolis, Minnesota. Poster No. P12003. Available: http://abstracts.aspb.org/pb2011/public/P12/P12003.html.

Technical Abstract: Phosphorus (P) is one of the most limiting macronutrients in soils for plant growth and development. White lupin (Lupinus albus) has evolved unique adaptation systems for growth in P-deficient conditions (-P) in soils including: 1) development of densely clustered determinant lateral roots called proteoid roots to increase root surface area; 2) root exudation of organic acids, protons, and acid phosphatases to aid in P uptake; and 3) induction of numerous -P responsive genes such as transporter (Pi, MATE, and ions), phosphatase, carbon, and lipid metabolism genes. However, the molecular mechanism of white lupin's adaptation to -P, especially regulation of gene expression, gene networks, and signaling pathways, remains largely unknown. In this study, we utilized RNA-Seq technology to assess global gene expression change in white lupin roots and leaves in response to -P stress. We have generated more than 143 million EST reads (76 bp) derived from roots and leaves under -P as well as +P (P-sufficient) controls. The EST reads were assembled into ~100,000 contigs with an average length of ~1100 bp. Digital gene expression analysis indicated the enrichment of previously known -P responsive genes among genes up-regulated in roots under -P. In addition, we newly identified numerous gene classes responsive to -P in roots and leaves including transcription factors, hormone genes, stress responsive genes, signaling genes, and microRNAs and their targets. This study is part of a continuing effort to elucidate the molecular mechanism of plant adaptation to -P stress and provides new insight into -P signal perception and transduction, global gene expression regulation, and its consequences on the final phenotypic change under P-deficient conditions.

Last Modified: 12/21/2014
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