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United States Department of Agriculture

Agricultural Research Service

Research Project: DISCOVERY AND DEVELOPMENT OF NATURAL PRODUCT FOR PHARMACEUTICAL AND AGRICHEMICAL APPLICATIONS

Location: Natural Products Utilization Research

Title: Quantitative determination of triterpene saponins and alkenated-phenolics from Labisia pumila using LC-UV/ELSD method and confirmation by LC-ESI-TOF

Authors
item Avula, Bharathi -
item Wang, Yan-Hong -
item Ali, Zulfiqar -
item Smillie, Troy -
item Khan, Ikhlas -
item Duke, Stephen

Submitted to: Planta Medica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 1, 2011
Publication Date: May 17, 2011
Citation: Avula, B., Wang, Y., Ali, Z., Smillie, T.J., Khan, I.A., Duke, S.O. 2011. Quantitative determination of triterpene saponins and alkenated-phenolics from Labisia pumila using LC-UV/ELSD method and confirmation by LC-ESI-TOF. Planta Medica. 77:1742-1748.

Interpretive Summary: A newly developed method was found for the determination of saponins and alkenated phenolics from the aerial parts and roots of Labisia pumila, a plant used in traditional Malaysian medicine. Ardisiacrispin A was found to be a major compound for all plant samples analyzed. The developed method is simple, economic and especially suitable for quality control analysis of products.

Technical Abstract: This study describes the first analytical method for the determination of saponins and alkenated-phenolics from the leaves, leaves/stems and roots of Labisia pumila using a HPLC-UV-ELSD method. The separation was achieved using a reversed phase column, PDA and ELS detection, and a water/acetonitrile gradient as the mobile phase. Analysis of various samples showed considerable variation of 0.11-2.46% for the major triterpenoid compound, ardisiacrispin A. LC-mass spectrometry method coupled with ESI is described for the identification of compounds in plant samples.

Last Modified: 7/30/2014