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Research Project:
GENETIC IMPROVEMENT OF FRUIT CROPS THROUGH FUNCTIONAL GENOMICS AND BREEDING
Location: Appalachian Fruit Research Laboratory: Innovative Fruit Production, Improvement and Protection
Title: Inheritance of plum pox virus resistance in transgenic plums
Authors
 | Ravelonandro, Michel - | |
Scorza, Ralph
| | Briard, Pascal - | | Lafargue, Briard - | | Renaud, Renee - |
Submitted to: Acta Horticulturae
Publication Type: Proceedings
Publication Acceptance Date: March 11, 2011
Publication Date: July 15, 2011
Citation: Ravelonandro, M., Scorza, R., Briard, P., Lafargue, B., Renaud, R. 2011. Inheritance of plum pox virus resistance in transgenic plums. Acta Horticulturae. 899:139-144.
Technical Abstract:
We have studied the heritability of the virus transgene engineered in 'HoneySweet' plum through different cross-hybridization with two commercial cultivars of Prunus domestica (Prunier d’Ente 303 and Quetsche 2906) and one wild species, P. spinosa 2862, rootstock using 'HoneySweet' plum as the pollen donor. Over all crosses, 46% of the progeny were transgenic. Chi square analyses indicated that the transgene was inherited as a single dominant allele. Transgenic hybrids were challenged with Plum pox virus (PPV), and resistance was analyzed through more than three dormancy cycles. Transgenic hybrids were highly resistant to sharka disease, while the untransformed sibling hybrids were susceptible to PPV. As in the 'HoneySweet' parent, silencing was shown to be the mechanism of resistance. These results confirmed the high potential of 'HoneySweet' plum for PPV resistance breeding programs.
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Last Modified: 05/22/2013
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