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United States Department of Agriculture

Agricultural Research Service

Research Project: REDUCING SOYBEAN YIELD LOSSES THROUGH GENETIC IMPROVEMENT

Location: Crop Genetics Research Unit

Title: Reaction of mid-southern U.S. southern cultivars to Bean pod mottle virus and Tobacco ringspot virus

Authors
item Skakiba, Ehsan -
item Chen, Pengyin -
item Gergerich, Rose -
item Li, Shuxian
item Shi, Ainong -

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 22, 2012
Publication Date: September 1, 2012
Citation: Skakiba, E., Chen, P., Gergerich, R., Li, S., Shi, A. 2012. Reaction of mid-southern U.S. southern cultivars to Bean pod mottle virus and Tobacco ringspot virus. Crop Science. 52(5):1980-1989.

Interpretive Summary: Bean pod mottle virus (BPMV) and Tobacco ringspot virus (TRSV) are two important viral pathogens causing reduction of seed yield and quality in soybean. The objective of this study was to screen modern soybean cultivars for resistance or tolerance to BPMV or TRSV. A total of 303 cultivars currently grown in midsouthern U.S. were screened in the greenhouse for their reactions to BPMV and TRSV. Each cultivar was inoculated with the viruses. No cultivar had resistance to BPMV; however, there was a range of tolerance to this virus among the cultivars. Tolerance to the virus is associated with less reduction in plant height and weight. There was no resistance to TRSV, and all cultivars showed bud blight (death of terminal buds) 10 days after inoculation. However, 55 cultivars recovered 5 weeks after inoculation with more than 80% of the plants growing out of the bud blight and forming new leaves. The recovered plants, although systemically infected, clearly showed tolerance to TRSV at this later stage. These results may be helpful to soybean producers in selecting tolerant cultivars to grow in areas where BPMV and/or TRSV may cause problems in production.

Technical Abstract: Bean pod mottle virus (BPMV) and Tobacco ringspot virus (TRSV) are two important viral pathogens causing reduction of seed yield and quality in soybean. There are various BPMV and TRSV isolates observed, but no host resistance reported so far. The objective of this study was to screen modern soybean cultivars for resistance or tolerance to BPMV or TRSV. A total of 303 cultivars currently grown in midsouthern U.S. were screened in the greenhouse for their reactions to BPMV and TRSV. Each cultivar was inoculated with two BPMV isolates [K-Ha1, representing the mild virulent isolate (subgroup II) and K-Ho1 representing severe reassortment isolate (I/II)] and two TRSV isolates (mild and severe). The results showed no resistance to BPMV; however, there was a range of tolerance to this virus among the cultivars. ELISA confirmed presence of BPMV in high and low tolerance cultivars. Tolerance to the virus is associated with less reduction in plant height and biomass; therefore, cultivars were classified into four groups: high, moderate, low, and very low tolerance based on =20, 25-45, 45-60, and =60% plant height reduction and =25, 25-50, 50-75, and =75% plant biomass reduction, respectively. There was no resistance to TRSV, and all cultivars showed bud blight 10 days after inoculation. However, 55 cultivars recovered 5 weeks after inoculation with more than 80% of the plants growing out of the bud blight and forming new leaves. The recovered plants, although systemically infected, clearly showed tolerance to TRSV at a later stage. These results may be helpful to soybean producers in selecting tolerant cultivars to grow in areas where BPMV and/or TRSV may cause problems in production.

Last Modified: 8/27/2014