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United States Department of Agriculture

Agricultural Research Service

Research Project: MANAGEMENT OF TEMPERATE FRUIT NUT AND SPECIALTY CROP GENETIC RESOURCES Title: Cryopreservation of strawberry genetic resources in Germany

Authors
item Hoefer, Monika -
item Reed, Barbara

Submitted to: Acta Horticulturae
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 15, 2011
Publication Date: February 4, 2012
Citation: Hoefer, M., Reed, B.M. 2012. Cryopreservation of strawberry genetic resources in Germany. Acta Horticulturae. 918:139-146.

Interpretive Summary: The National German Strawberry Genebank includes 369 cultivars and the active field collection in Dresden-Pillnitz also contains 318 Fragaria wild species accessions. Conservation of clonal crops requires safety duplication. An earlier calculation of the effort required to establish and maintain a safety duplication of the whole collection with tissue culture plants indicated that it is too labor intensive to be maintained. The development of an effective method for cryopreservation in liquid nitrogen would be more cost effective. Four cryopreservation protocols were tested. All four techniques were equally effective for the strawberry cultivars tested, however the Fragaria species grew best with only one of the techniques. This technique will be the standard method for cryopreserving the strawberry collection. Twenty strawberry types are now in the permanent cryopreservation collection.

Technical Abstract: The National German Strawberry Genebank includes 369 cultivars and the active field collection in Dresden-Pillnitz also contains 318 Fragaria wild species accessions. Conservation of clonal crops requires safety duplication. An earlier calculation of the effort required to establish and maintain a safety duplication of the whole collection with in vitro cold storage indicated that it is too labor intensive to be maintained. The development of an effective method for cryopreservation is required for cost effective long-term storage. Four distinct cryopreservation protocols were tested: Two Plant Vitrification Solution 2 (PVS2) protocols, encapsulation dehydration and controlled rate cooling. All four techniques were equally effective for the cultivars tested, however the Fragaria species had significantly better regrowth with the PVS2 vitrification protocol that included 14 d alternating-temperature cold acclimation (16h at -1°C and 8h at 22°C). This PVS2 vitrification protocol with cold acclimation will be applied as the standard method for Fragaria cultivars and wild species. Twenty genotypes are now in the permanent cryopreservation collection.

Last Modified: 11/20/2014
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