Technical Abstract: Thirty Plant Introductions (PIs) from the USDA-ARS National Plant Germplasm System were evaluated for resistance to Cercospora beticola in an artificially produced epiphytotic at the Saginaw Valley Research and Extension Center near Frankenmuth, MI. Internal controls included a susceptible commercial variety, 'CE', and a resistant variety, 'ACH 355'. The field was planted April 14. The nursery was inoculated once with a liquid spore suspension of Cercospora beticola on July 1. Visual evaluations on the plot, producing a disease index (DI) on a scale from 0 (no disease) to 10 (plant dead), were made on five dates, with the peak of the epidemic occurring between the fourth and fifth dates. After the fifth date, regrowth of several PIs was visible, so ratings started to go down. At our fourth evaluation, means of the resistant and susceptible internal control were 3.2 and 5.5, respectively, across the nursery. In 2009, these means were 2.5 and 5.3, respectively. Means of the PIs ranged from 5.0 to 7.7. An analysis of variance on the disease indices determined that there were significant differences among entries (P=0.05) on all five dates of evaluation. Two accessions had average disease ratings significantly lower than the susceptible control at the second and third rating dates, but were not signficantly different from the susceptible control by the end of the rating period. In contrast, two accessions were observed that had significantly higher average disease ratings than the susceptible check on three of the five rating dates.