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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC AND GENOMIC APPROACHES TO IMPROVE PEANUT AND CORN RESISTANCE TO DISEASE AND AFLATOXIN CONTAMINATION Title: An Integrated Linkage Map for Cultivated Peanut Derived from Two RILs Populations

Authors
item Guo, Baozhu
item Qin, Hongde -
item Feng, Suping -
item Chen, Charles
item Knapp, Steven -
item Culbreat, Albert -
item He, Guohao -
item Wang, Ming
item Zhang, Xingyu -
item Holbrook, C

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: December 10, 2010
Publication Date: January 15, 2011
Citation: Guo, B., Qin, H., Feng, S., Chen, C.Y., Knapp, S., Culbreat, A., He, G., Wang, M.L., Zhang, X., Holbrook Jr, C.C. 2011. An Integrated Linkage Map for Cultivated Peanut Derived from Two RILs Populations. Plant and Animal Genome Conference. San Diego January 15-19, 2011.

Technical Abstract: A comparable integrated map for cultivated peanut (Arachis hypogaea L.) was constructed from the integration of two recombinant inbred line (RIL) populations in which two runner type cultivars, one Spanish type cultivar and one breeding line derived from a cross of Virginia type and hirsuta type were used as parents. Simple sequence repeat (SSR) markers from three sources were used: published SSR markers, newly developed SSR markers derived from expressed sequence tags (EST) and bacterial artificial chromosome (BAC) end-sequences. A total of 321 markers were mapped on this integrated map which covered 1,335.0 cM with 20 linkage groups. Combining information from duplicated loci between linkage groups and comparing with published diploid maps, 6 homoeologous pairs were defined and 16 linkage groups (A1 to A10, B1 to B4, B7, and B8) were aligned to corresponding A-subgenome and B-subgenome of diploid progenitors. Linkage groups for the A-subgenome covered 796.0 cM with 199 loci, and for the B subgenome covered 539.0 cM with 122 loci. One reciprocal translocation was confirmed in the tetraploid cultivated peanut genome. Several rearrangements of chromosome structures were observed by comparing to cultivated peanut maps. There were 42, 30 and 46 collinear markers between this integrated map and three published cultivated linkage maps, respectively. High consistence with cultivated peanut maps derived from different populations supports this integrated map as a reliable reference map which can be used for detecting QTLs, molecular breeding, and gene discovery.

Last Modified: 10/24/2014
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