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United States Department of Agriculture

Agricultural Research Service

Research Project: UTILIZATION OF GENOMICS FOR IMPROVING PRODUCTION TRAITS IN COOL AND COLD WATER AQUACULTURE

Location: Cool and Cold Water Aquaculture Research

Title: A microsatellite linkage map of striped bass (Morone saxatilis)

Authors
item Liu, Sixin
item Rexroad, Caird
item Couch, Charlene -
item Cordes, Jan -
item Reece, Kimberly -
item Sullivan, Craig -

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: December 1, 2010
Publication Date: January 15, 2011
Citation: Liu, S., Rexroad III, C.E., Couch, C.R., Cordes, J.R., Reece, K.S., Sullivan, C.V. 2011. A microsatellite linkage map of striped bass (Morone saxatilis) [abstract]. Plant and Animal Genome Conference. p. 628.

Technical Abstract: Striped bass (Morone saxatilis) is of great importance for fisheries and aquaculture in the US. To construct a linkage map of striped bass, 480 microsatellite markers were screened for polymorphism among three parents of two half-sib mapping families that shared a common dam. A total of 289 markers were informative in one or both of the two mapping families, and both families (93 and 94 offspring, respectively) were genotyped for the informative markers. Twenty-six linkage groups were assembled, and only two of the 289 informative markers remain unlinked. The sex-averaged map spans 1701.6 cM with an average marker density of 5.99 cM per marker. The male genetic map length is 1967.3 cM, and the female genetic map length is 1613.1 cM; therefore, the overall male:female recombination ratio is 1.22:1. The sequences of striped bass microsatellite markers were compared by BLAST searches with the three-spined stickleback (Gasterosteus aculeatus) genome. Among 285 striped bass microsatellite markers, 169 (59%) were mapped onto stickleback chromosomes or scaffolds, and the syntenic relationship between these two fish genomes were identified for the first time. This is the first linkage map for any of the Morone species and will facilitate the discovery of genes affecting biochemical processes underlying important traits.

Last Modified: 8/27/2014
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