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United States Department of Agriculture

Agricultural Research Service

Research Project: MINING THE GENOME OF RHIPICEPHALUS MICROPLUS TO DEVELOP NOVEL CONTROL TECHNOLOGY AND VACCINES

Location: Tick and Biting Fly Research

Title: Rhipicephalus microplus strain Deutsch, 10 BAC clone sequences

Authors
item Moolhuijzen, P. -
item Guerrero, Felix
item Bellgard, M -
item Schibeci, D -
item Hunter, A -
item Barrero, R -
item Rodriguez-Valle, M -
item Lew-Tabor, A -

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: July 9, 2010
Publication Date: June 29, 2011
Citation: Moolhuijzen, P., Guerrero, F., Bellgard, M., Schibeci, D., Hunter, A., Barrero, R., Rodriguez-Valle, M., Lew-Tabor, A. 2011. Rhipicephalus microplus strain Deutsch, 10 BAC clone sequences. National Center for Biotechnology Information (NCBI).

Interpretive Summary: The cattle tick, Rhipicephalus (Boophilus) microplus, has a genome over 2.4 times the size of the human genome, and with over 70% of repetitive DNA, this genome would prove very costly to sequence at today's prices and difficult to assemble and analyze. We used labeled DNA probes from the coding regions of protein coding regions of interest to isolate 10 BAC clones from the R. microplus BAC library 1 from the Deutsch strain. These BACs were sequenced and assembled with BAC clone Accession Numbers HM748958-HM748967.

Technical Abstract: The cattle tick, Rhipicephalus (Boophilus) microplus, has a genome over 2.4 times the size of the human genome, and with over 70% of repetitive DNA, this genome would prove very costly to sequence at today's prices and difficult to assemble and analyze. We used labeled DNA probes from the coding regions of protein coding regions of interest to isolate 10 BAC clones from the R. microplus BAC library 1 from the Deutsch strain. These BACs were sequenced and assembled with BAC clone Accession Numbers HM748958-HM748967.

Last Modified: 4/19/2014
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