ARS | Publication request: Rhipicephalus (Boophilus) microplus strain Deutsch, 5 BAC clone sequencing, including two encoding Cytochrome P450s and one encoding CzEst9 carboxylesterase

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: May 6, 2010
Publication Date: June 11, 2010
Citation: Inman, J.M., Caler, E., Guerrero, F. 2010. Rhipicephalus (Boophilus) microplus strain Deutsch, 5 BAC clone sequencing, including two encoding Cytochrome P450s and one encoding CzEst9 carboxylesterase. National Center for Biotechnology Information (NCBI).

Interpretive Summary: The cattle tick, Rhipicephalus (Boophilus) microplus, has a genome over 2.4 times the size of the human genome, and with over 70% of repetitive DNA, this genome would prove very costly to sequence at today's prices and difficult to assemble and analyze. BAC clones give insight into the genome structure and gene coding regions of an organism. This reports the complete BAC sequence for BAC clones Bm-74-F12, Bm-77-J9, Bm-129-N14, Bm-66-M7, and Bm-77-G20 with Accession numbers of HM193853-HM193857, respectively. BACs 66-M7 and 77-G20 contain coding regions for Cytochrome P450s. BAC 129-N14 contains the coding region for the pyrethroid-hydrolyzing carboxylesterase CzEst9. BACs 77-J9 and 74-F12 were randomly chosen and contain predominantly repetitive DNA.

Technical Abstract: The cattle tick, Rhipicephalus (Boophilus) microplus, has a genome over 2.4 times the size of the human genome, and with over 70% of repetitive DNA, this genome would prove very costly to sequence at today's prices and difficult to assemble and analyze. BAC clones give insight into the genome structure and gene coding regions of an organism. This reports the complete BAC sequence for BAC clones Bm-74-F12, Bm-77-J9, Bm-129-N14, Bm-66-M7, and Bm-77-G20 with Accession numbers of HM193853-HM193857, respectively. BACs 66-M7 and 77-G20 contain coding regions for Cytochrome P450s. BAC 129-N14 contains the coding region for the pyrethroid-hydrolyzing carboxylesterase CzEst9. BACs 77-J9 and 74-F12 were randomly chosen and contain predominantly repetitive DNA.