ARS | Publication request: Molecular and genetic characterization of Hessian fly-resistance genes in synthetic hexaploid wheat

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: October 26, 2010
Publication Date: December 10, 2010
Citation: Xu, S.S., Yu, G., Cai, X., Harris, M. 2010. Molecular and genetic characterization of Hessian fly-resistance genes in synthetic hexaploid wheat. Meeting Abstract. 157.

Technical Abstract: Synthetic hexaploid wheat (SHW), derived from crosses between tetraploid wheat (Triticum turgidum) and Aegilops tauschii, has proven to be an excellent source of resistance genes for various diseases and pests in wheat (T. aestivum). We previously evaluated a large collection of SHW lines for resistance to Hessian fly and identified 43 SHW lines with resistance (H) genes derived from Ae. tauschii. This research was conducted to characterize the H genes in these resistant SHW lines by haplotype analysis, allelism tests, and molecular mapping. Haplotype analyses with molecular markers closely-linked to five H genes (H13, H22, H23, H26, and H32) derived from Ae. tauschii suggest that some of the SHW lines contain H genes different from H13, H22, H24, H26, and H32. Through allelism tests, two of the SHW lines were verified to carry H26 and H13, respectively. Molecular mapping demonstrated that H26 and H32 could be either different alleles at the same gene locus or two different, but tightly linked H genes on chromosome arm 3DL. Also we mapped a recessive Hessian fly-resistance gene on chromosome 1D in one of the SHW lines. The newly developed STS (sequence tagged site) and microsatellite markers closely-linked to H26, H32, and the recessive gene in this study are currently being used to assist deployment of these genes into adapted bread wheat germplasm.