ARS | Publication request: Analysis of soybean flowering-time genes

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: November 16, 2009
Publication Date: January 9, 2010
Citation: Thakare, D., Kumudini, S., Dinkins, R.D. 2010. Analysis of soybean flowering-time genes. Plant and Animal Genome XVIII Conference. P758.

Technical Abstract: Control of soybean flowering time is important for geographic adaptation, and maximizing yield. RT-PCR analysis was performed using primers synthesized for a number of putative flowering-time genes based on homology of soybean EST and genomic sequences to Arabidopsis genes. RNA for cDNA synthesis was isolated from plant samples harvested every three hours over a 48 hr period under long day (20h light/ 4h dark) and short day (12h light/ 12 dark) beginning at day 8. The time was selected based on results of reciprocal transfer experiments that suggest that the pre-inductive photoperiod-sensitive phase responsible for inducing flowering in the soybean genotypes tested was perceived as early as 5-8 d post planting. The gene expression results in this study were similar to those of flowering-time genes found in other short-day species, suggesting that the selected genes correspond to the soybean flowering-time orthologues.