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United States Department of Agriculture

Agricultural Research Service

Research Project: DIET, INFLAMMATION AND PREVENTION OF CHRONIC DISEASE

Location: Immunity and Disease Prevention Research Unit

Title: Modulation of blood cell gene expression by DHA supplementation in hypertriglyceridemic men

Authors
item Dawson, Kevin -
item Zhao, Ling -
item Adkins, Yuriko
item Rodriguez, Raymond -
item Kelley, Darshan
item Hwang, Daniel

Submitted to: Journal of Nutritional Biochemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 8, 2011
Publication Date: July 18, 2011
Repository URL: http://www.sciencedirect.com/science/article/pii/S0955286311000933
Citation: Dawson, K., Zhao, L., Adkins, Y.C., Rodriguez, R.L., Kelley, D.S., Hwang, D.H. 2011. Modulation of blood cell gene expression by DHA supplementation in hypertriglyceridemic men. Journal of Nutritional Biochemistry. Available: http://www.sciencedirect.com/science/article/pii/S0955286311000933

Interpretive Summary: To determine the effect of DHA supplementation on the global gene expression pattern, we performed Affymetrix GeneChip microarray analysis of blood cells (treated with lipopolysaccharide (LPS) or vehicle) drawn before and after the supplementation of DHA from the hypertriglyceridemic men who participated in that study. DHA supplementation significantly suppressed the expression of LDL receptor (LDLR) and cathepsin L 1 (CTSL1), both of which were also up-regulated by LPS. DHA supplementation also suppressed oxidized LDL (lectin-like) receptor 1 (OLR1). However, LPS did not induce OLR1 mRNA expression. Enrichment with Gene Ontology categories demonstrated that the genes related to transcription factor activity, immune, host defense and inflammatory responses, were inversely regulated by LPS and DHA. These results provide supporting evidence for the anti-inflammatory effects of DHA supplementation, and reveal previously unrecognized genes that are regulated by DHA, and are associated with risk factors of cardiovascular diseases

Technical Abstract: Our previous study with docosahexaenoic acid (DHA) supplementation to hypertriglyceridemic men showed that DHA reduced several risk factors for CVD, including the plasma concentration of inflammatory markers. To determine the effect of DHA supplementation on the global gene expression pattern, we performed Affymetrix GeneChip microarray analysis of blood cells (treated with lipopolysaccharide (LPS) or vehicle) drawn before and after the supplementation of DHA from the hypertriglyceridemic men who participated in that study. Genes that were significantly differentially regulated by the LPS treatment and DHA supplementation were identified. Differential regulation of 18 genes was then verified by quantitative RT-PCR. Both microarray and qRT-PCR data showed that DHA supplementation significantly suppressed the expression of LDL receptor (LDLR) and cathepsin L 1 (CTSL1), both of which were also up-regulated by LPS. DHA supplementation also suppressed oxidized LDL (lectin-like) receptor 1 (OLR1). However, LPS did not induce OLR1 mRNA expression. Enrichment with Gene Ontology categories demonstrated that the genes related to transcription factor activity, immune, host defense and inflammatory responses, were inversely regulated by LPS and DHA. These results provide supporting evidence for the anti-inflammatory effects of DHA supplementation, and reveal previously unrecognized genes that are regulated by DHA, and are associated with risk factors of cardiovascular diseases.

Last Modified: 4/16/2014
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