Author
Yakabe, Lani | |
MACCREE, M - University Of California | |
SUDARSHANA, P - Monsanto Corporation | |
McClean, Ali | |
PARKER, S - US Department Of Agriculture (USDA) | |
Wechter, William - Pat | |
PRESTING, G - University Of Hawaii | |
Kluepfel, Daniel |
Submitted to: Journal of General Plant Pathology
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 1/16/2012 Publication Date: 2/15/2012 Citation: Yakabe, L.E., Maccree, M.M., Sudarshana, P., Mcclean, A.E., Parker, S.R., Wechter, W.P., Presting, G., Kluepfel, D.A. 2012. Novel primers for detection of genetically diverse virulent Agrobacterium tumefaciens bv1 strains. Journal of General Plant Pathology. DOI: 10.1007/s10327-012-0364-z. Interpretive Summary: Technical Abstract: Novel primers were developed to amplify a 243 bp fragment of an intergenic region between gene5 and tms2 on the T-DNA of Agrobacterium tumefaciens. These primers exhibit 100% positive correlation with strain virulence, 100% negative correlation with avirulence and did not generate extraneous bands, facilitating robust real-time PCR detection. |