ARS | Publication request: Mapping Resistance to Peanut Mottle Virus in Common Bean

Submitted to: Bean Improvement Cooperative Annual Report
Publication Type: Proceedings
Publication Acceptance Date: February 25, 2010
Publication Date: March 21, 2010
Citation: Larsen, R.C., Miklas, P.N. 2010. Mapping Resistance to Peanut Mottle Virus in Common Bean. Bean Improvement Cooperative Annual Report. 53:50-51.

Technical Abstract: Peanut mottle virus (PeMoV) causes severe symptoms of systemic vein necrosis in susceptible snap beans, resembling those caused by Bean common mosaic necrosis virus. During 2008 and 2009, PeMoV was identified in snap bean fields in Frio County, TX. In lieu of the recent PeMoV outbreak, our objective was to identify available sources of PeMoV resistance, and to characterize and locate the gene(s) on the Phaseolus core map. A RIL population consisting of 75 F9-derived lines from the cross between BelNeb-RR-1 (PeMoV-resistant) and A55 (PeMoV-susceptible), ( BA population), and a second RIL population consisting of F5:7 RILs derived by from a cross between the landrace cultivar G122 (resistant) and A55 (AG population) were inoculated with PeMoV. Resistance to PeMoV derived from BelNeb-RR-1 was mapped to linkage group 3 on the Phaseolus core map. Similarly, resistance in the AG population was determined also to be located on LG 3. Integration of these groups on the core map suggested the gene was located in the vicinity of the bc-1-2 gene conferring resistance to Bean common mosaic virus and Bean common mosaic necrosis virus. To examine this relationship further, SCAR marker SBD5.1330 linked with bc-12 was mapped in the BA population. Results showed that resistance to PeMoV derived from BelNeb RR-1 is directly linked (0 recombinants) to SBD5.1330 as determined by co-segregation with the marker. SBD5.1330 was also determined to be present in G122. However, based on an extended host range response, it was determined that resistance to PeMoV does not appear to be dependent on bc-12.