ARS | Publication request: Variation in Anthocyanin Content of Wild Black Raspberry for Breeding Improved Cultivars

Submitted to: Annual Meeting Horticultural Society
Publication Type: Abstract Only
Publication Acceptance Date: August 5, 2010
Publication Date: August 5, 2010
Citation: Dossett, M., Lee, J., Finn, C.E. 2010. Variation in anthocyanin content of wild black raspberry for breeding improved cultivars. Annual Meeting Horticultural Society. Abstract 4377.

Technical Abstract: Because of its intense anthocyanin pigments, black raspberry (Rubus occidentalis L.) has a long history of use as a natural colorant and dye. Recent studies showing black raspberries to be a rich source of anthocyanins and other dietary phytochemicals has led to renewed interest in breeding new, better adapted cultivars that meet the demands of these markets. Because of this, anthocyanin content is a critical indicator of fruit quality for processed markets. While previous studies characterizing black raspberry anthocyanins have focused on existing cultivars comprising a narrow genetic base, progress in breeding new cultivars will rely on the use of new germplasm sources. Using high performance liquid chromatography/diode array detector/ion trap mass spectrometer, we examined anthocyanin content and profiles in the juice of fruit from black raspberry seedlings representing 78 wild populations from across the species' native range. Anthocyanin profiles were similar to those previously reported; however, total anthocyanin content varied widely, with individual clones ranging from less than one fourth to nearly three times the anthocyanin concentration of the industry standard, ‘Munger’. Genetic diversity for anthocyanin content is present in recently collected wild black raspberry germplasm and should be carefully evaluated when using this material for breeding improved cultivars.