Location: Children's Nutrition Research Center
Title: Ferric reductase activity and PsFRO1 sequence variation in pisum sps Authors
|Klein, Melinda -|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: October 10, 2009
Publication Date: October 28, 2009
Citation: Klein, M.A., Grusak, M.A. 2009. Ferric reductase activity and PsFRO1 sequence variation in pisum sps [Abstract]. North America Pulse Improvement Association Biennial Meeting. p. 9. Technical Abstract: Physiological studies in pea (Pisum sativum) suggest that the reduction of iron (Fe) is the rate-limiting physiological process in Fe acquisition by dicotyledonous plants. Previous molecular work suggests that ferric reductase activity is regulated at both the transcriptional and post-translational levels. In order to further dissect the regulation of ferric reductase transcription and activity, we are conducting a survey of 32 pea accessions derived from single-seed descent (obtained from the USDA Germplasm Collection, Pullman, WA). Plants were grown under low and high Fe conditions (0.5 and 15 uM Fe[III]-EDDHA) and analyzed for root ferric reductase activity. In addition, PsFRO1 was isolated and sequenced from all lines. Across the accessions, ferric reductase activity was variable and several polymorphisms were identified in PsFRO1. This variation will be used to discuss the genetic and functional attributes that contribute to the regulation of iron homeostasis at the whole-plant level in pea.