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United States Department of Agriculture

Agricultural Research Service

Research Project: MANAGEMENT OF TEMPERATE FRUIT NUT AND SPECIALTY CROP GENETIC RESOURCES Title: In vitro cold-storage duration of sour cherry (Prunus cerasus L) shoots is affected by carbon source and nitrogen concentration

Authors
item Kovalchuk, Irina -
item Nasibulinal, Albina -
item Reed, Barbara

Submitted to: Acta Horticulturae
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 15, 2011
Publication Date: February 2, 2012
Citation: Kovalchuk, I., Nasibulinal, A., Reed, B.M. 2012. In vitro cold-storage duration of sour cherry (Prunus cerasus L) shoots is affected by carbon source and nitrogen concentration. Acta Horticulturae. 918:167-176.

Interpretive Summary: Cold storage of fruit crop plant as tissue cultures is useful for preservation of heritage or commercial cultivars. Shoot cultures of three sour cherry cultivars were cold stored at 4°C in either tissue culture bags or glass jars. Combinations of sugars were tested in growth medium. Nitrogen at 100%, 50% or 25% of the normal concentration was also tested. Shoot cultures of the three cherry cultivars could be stored for over 30 months at refrigerator temperatures and remained in good condition in some treatments. Sucrose was the best sugar for the three genotypes and allowed storage for up to three years. Shoots stored on mannitol survived for only 6 to 12 months while the combination of mannitol and sucrose extended storage to 30 months for two cultivars. Fifty-seven sour cherry types were cold stored in tissue culture bags on 3% sucrose growth medium and remained in good condition for 13 to 30 months. The 68 accessions of the sour cherry collection are now stored in tissue culture bags with sucrose.

Technical Abstract: In vitro cold storage of fruit crop germplasm is useful for preservation of heritage or commercial cultivars. Shoot cultures of sour cherry (Prunus cerasus L.) cultivars Dolgozdannaya, Moya Radost and Zukovskaya, were cold stored at 4°C in either five-section tissue-culture bags or in 150 ml glass jars. Carbon sources (3% sucrose, 2% or 3% mannitol, or 2% sucrose + 2% mannitol) were tested in Murashige and Skoog (MS) medium with or without plant growth regulators (PGRs). Nitrate nitrogen at 100%, 50% or 25% of the normal MS concentration was also tested. Shoot cultures of the three cherry cultivars could be stored for over 30 months at 4 °C and remained in good condition in some treatments. There was significant variation in the storage duration with significant interactions of the cultivar, treatment, and container. Sucrose was the best carbon source for all three genotypes and allowed storage for more than 30 months. Shoots stored on 2% or 3% mannitol survived for only 6 to 12 months while the combination of 2% mannitol and 2% sucrose extended storage to 30 months. The addition of abscisic acid to 3% sucrose MS medium significantly decreased storage duration. Fifty-seven accessions of sour cherry germplasm were stored in tissue culture bags on 3% sucrose MS medium without PGRs and remained in good condition for 13 to 30 months. The 68 accessions of the in vitro P. cerasus germplasm collection are now stored in tissue-culture bags with MS medium, PGRs, and 3% sucrose.

Last Modified: 11/23/2014
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