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United States Department of Agriculture

Agricultural Research Service

Research Project: MOLECULAR, CELLULAR, AND REGULATORY ASPECTS OF OBESITY DEVELOPMENT IN CHILDREN

Location: Children's Nutrition Research Center

Title: Intermittent bolus feeding has a greater stimulatory effect on protein synthesis in skeletal muscle than continuous feeding in neonatal pigs

Authors
item Gazzaneo, Maria -
item Murgas-Torrazza, Roberto -
item Suryawan, Agus -
item Orellana, Renan -
item Srivastava, Neeraj -
item Nguyen, Hanh -
item Wilson, Fiona -
item Fiorotto, Marta -
item Davis, Teresa -

Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: February 14, 2010
Publication Date: April 24, 2010
Repository URL: http://www.fasebj.org/cgi/content/meeting_abstract/24/1_MeetingAbstracts/327.3?maxtoshow=&hits=10&RESULTFORMAT=&fulltext=suryawan&searchid=1&FIRSTINDEX=0&volume=24&issue=1_MeetingAbstracts&resourcetype=HWCIT
Citation: Gazzaneo, M.C., Murgas-Torrazza, R., Suryawan, A., Orellana, R.A., Srivastava, N., Nguyen, H.V., Wilson, F.A., Fiorotto, M.L., Davis, T.A. 2010. Intermittent bolus feeding has a greater stimulatory effect on protein synthesis in skeletal muscle than continuous feeding in neonatal pigs [abstract]. Federation of American Societies for Experimental Biology Conference, Session: Animal research models in macronutrient metabolism I, April 24-28, 2010, Anaheim, California. 24: 327.3.

Technical Abstract: Feeding by orogastric tube, using either continuous or intermittent bolus delivery, is common for infants for whom normal feeding is contraindicated. To determine the impact of different feeding modalities on muscle protein anabolism, neonatal pigs (5–7 day old) received a balanced enteral formula either by intermittent bolus (B, n=7) every four hours, or were continuously fed (C, n=5). Fractional protein synthesis rates and translational control mechanisms in skeletal muscle were examined at 0, 24, and 25.5 hours. Plasma amino acid and insulin levels were modestly increased in the continuously fed group and in the bolus group mimicked the pulsatile pattern of meal feeding. Feeding increased protein synthesis by 53% in the continuous group, by 63% in the bolus group before a meal, and by 126% after a bolus feed. The activation of eukaryotic initiation factor (eIF) 4E binding protein (4EBP1), rp S6 kinase 1 (S6K1), protein kinase B (PKB), dissociation of 4EBP1-eIF4E complex, and formation of the active eIF4E-eIF4G complex were unaffected by continuous feeding, but were increased more than 10 fold after a bolus meal. These results suggest that intermittent bolus feeding increases muscle protein synthesis to a greater extent than continuous feeding by stimulating translation initiation.

Last Modified: 9/22/2014
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