Technical Abstract: The germplasm base of strawberries is restricted. The major cultivated strawberry species, Fragaria ananassa, originated about 250 years ago when South American F. chiloensis subsp. chiloensis forma chiloensis and North American F. virginiana subsp. virginiana accidentally hybridized in European gardens. Since that time, only a handful of native clones have been utilized by breeders. As a novel way to expand the germplasm base of the strawberry, we pre-selected native clones of F. virginiana and F. chiloensis for a wide range of horticulturally important characteristics and then reconstructed F. ananassa by crossing superior clones of each. Before crossing between species, we undertook one round of selection within species to maximize diversity. Reconstruction appeared to be an effective method of strawberry improvement, as superior families and individuals were identified that had outstanding vigor, high productivity, seed set, fruit color and firmness. None of the fruit were of commercial size, but one reconstruction family, FVC 11 [(F. virginiana Frederick 9 x LH 50-4) x (F. chiloensis Scotts Creek x 2 MAR 1A)], had individuals with fruit weights of almost 20 g.