Location: Hard Winter Wheat Genetics Research Unit
Title: Mapping Quantitative Trait Loci for Quality Factors in an Inter-Class Cross of US and Chinese Wheat Authors
|Sun, Xiaochun -|
|Marza, Felix -|
|Ma, Hongxiang -|
|Carver, Brett -|
Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: November 23, 2009
Publication Date: December 13, 2009
Citation: Sun, X., Marza, F., Ma, H., Carver, B., Bai, G. 2009. Mapping Quantitative Trait Loci for Quality Factors in an Inter-Class Cross of US and Chinese Wheat. Theoretical and Applied Genetics. 120:1041-1051. Interpretive Summary: Wheat quality factors are important in determining the suitability of wheat for end-use products and economic value, and they are major targets for marker-assisted breeding. A recombinant inbred population from a cross between the Chinese hard red wheat Ning7840 and the US soft red winter wheat Clark were evaluated for quality traits including grain protein content (GPC), test weight (TW), kernel weight (KW), and kernel diameter (KD), and hardness index (HI) at three Oklahoma locations from 2001 to 2003. A genetic map was constructed to identify quantitative trait loci (QTLs) responsible for these traits. Eight QTLs for TW, seven for KW, six for KD, two each for GPC and the HI measured by near-infrared reflectance (NIR) spectroscopy, and four for the HI measured by the single kernel characterization system. A QTL for HI was located at the Pinb-D1 gene for hardness on chromosome 5DS. The consistency of quality factor QTLs across environments reveals their potential for marker-assisted selection.
Technical Abstract: Wheat quality factors are critical in determining the suitability of wheat (Triticum aestivum L.) for end- use product and economic value, and they are prime targets for marker-assisted selection. Objectives of this study were to identify quantitative trait loci (QTLs) that ultimately influence wheat market class and milling quality. A population of 132 F12 recombinant inbred Iines (RILs) was derived by single-seed descent from a cross between the Chinese hard wheat line Ning7840 and the soft wheat cultivar CIark and grown at three Oklahoma locations from 2001 to 2003. Milling factors such as test weight (volumetric grain weight, TW), kernel weight (KW), and kernel diameter (KD) and market class factors such as wheat grain protein content (GPC) and kernel hardness index (HI) were characterized on the basis of a genetic map constructed from 367 SSR and 241 AFLP markers covering all 21 chromosomes. Composite interval mapping identified eight QTLs for TW, seven for KW, six for KD, two each for GPC and HI measured by near-infrared reflectance (NIR) spectroscopy, and four for HI measured by single kernel characterization system. Positive phenotypic correlations were found among milling factors. Consistent co-localized QTLs were identified for TW, KW, and KD on the short arms of chromosomes 5A and 6A. A common QTL was identified for TW and KD on the long arm of chromosome 5A. A consistent major QTL for HI peaked at the Pinb-D1 Iocus on the short arm of chromosome 5D and explained up to 85% of the phenotypic variation for hardness. We identified QTLs for GPC on 4B and the short arm of 3A chromosomes. The consistency of quality factor QTLs across environments reveals their potential for marker- assisted selection.