ARS | Publication request: Transcriptomes of Lolium/Schedonorus/Festuca Species

Submitted to: International Symposium on Fungal Endophytes of Grasses
Publication Type: Abstract Only
Publication Acceptance Date: April 30, 2010
Publication Date: June 28, 2010
Citation: Dinkins, R.D., Arnaoudova, E., Hesse, U., Nagabhyru, P., Moore, N., Liu, J., Jaromczyk, J., Zeng, Z., Liu, J., Zhao, P.X. 2010. Transcriptomes of Lolium/Schedonorus/Festuca Species. International Symposium on Fungal Endophytes of Grasses.p. 27.

Technical Abstract: ESTs from normalized cDNA libraries of tall fescue with Neotyphodium coenophialum and meadow fescue with Epichloë festucae have been sequenced. The meadow fescue libraries were from RNA isolated from immature tillers of meadow fescue symbiotic with E. festucae, displaying normal inflorescences and from inflorescences exhibiting the fruiting structures (stromata) of E. festucae. The tall fescue sequencing was from a normalized cDNA library from tall fescue (symbiotic with N. coenophialum) root mRNA that was isolated from combined plants subjected to differing drought stress treatments. A total of 128,189 EST sequences were obtained and have been submitted to NCBI. Following the removal of the sequences that mapped to the E. festucae genome, assembly of the plant reads, together with those of Festuca and Lolium spp from NCBI, resulted in 42,154 unigenes. The sequences were annotated by BLASTX against 1) the Gene Ontology (http://www.geneontology.org/) sequence database and 2) the uniprot trembl database (http://www.ebi.ac.uk/uniprot/). We have used the database to annotate and compare whole genome expression in meadow fescue infected with E. festucae with normal inflorescences and from inflorescences containing stromata using Illumina high throughput sequencing.