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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC AND GENOMIC APPROACHES TO IMPROVE PEANUT AND CORN RESISTANCE TO DISEASE AND AFLATOXIN CONTAMINATION Title: Evaluation of the expression of genes associated with resistance to Aspergillus flavus colonization and aflatoxin production in different maize lines

item Fountain, Jake
item Chen, Xhi-Yuan -
item Scully, Brian
item Kemerait, Robert -
item Lee, Dewey -
item Guo, Baozhu

Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: April 1, 2010
Publication Date: June 1, 2010
Citation: Fountain, J.C. Chen, X.Y. Scully, B.T. Kemerait, R.C. Lee, D.L. Guo, B. 2010. Evaluation of the expression of genes associated with resistance to Aspergillus flavus coloization and aflatoxin production in different maize lines. Phytopathology. 100:537.

Technical Abstract: Aflatoxins are carcinogenic toxic compounds produced by Aspergillus flavus during infection of crops including maize (Zea mays L.). Contamination of maize with aflatoxin is exacerbated by late season drought stress. Previous studies have implicated numerous resistance-associated proteins (RAPs) that may be responsible for resistance to A. flavus colonization and aflatoxin accumulation. This study examined the expression of three genes encoding RAPs, ZmPR-10 (PR-10), glyoxalase I (GLX-I), and a 14-kDa trypsin inhibitor (TI-14), in different maize genotypes under drought stressed and irrigated conditions to determine their potential utility as molecular markers for germplasm screening and evaluation utilizing quantitative real-time PCR. Results suggested that drought stress during kernel development affected gene expressions differently in different genotypes. Results were generally consistent with expectations in that RAP-coding gene expressions correlated well to known resistant traits of the examined genotypes. However, more genotypes should be studied in order to apply these genes’ expression as selection markers.

Last Modified: 11/27/2015