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ARS Home » Plains Area » Bushland, Texas » Conservation and Production Research Laboratory » Livestock Nutrient Management Research » Research » Publications at this Location » Publication #251659
Title: Expression of phosphate transporter in small intestine, kidney, and parotid salivary gland of cattle fed differing levels of phosphorus from wet distiller's grains

Author
item FOOTE, ANDREW - Tarleton State University
item LAMBERT, BARRY - Tarleton State University
item BRADY, J - Texas Agrilife Research
item BROWN, MIKE - West Texas A & M University
item OSTERSTOCK, JASON - Texas Agrilife Research
item MACDONALD, JIM - Texas Agrilife Research
item Cole, Noel

Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: 5/7/2010
Publication Date: 7/12/2010
Citation: Foote, A.P., Lambert, B.D., Brady, J.A., Brown, M.S., Osterstock, J.B., MacDonald, J.C., Cole, N.A. 2010. Expression of phosphate transporter in small intestine, kidney, and parotid salivary gland of cattle fed differing levels of phosphorus from wet distiller's grains [abstract]. Journal of Animal Science Supplement. 88:E-Supplement 2, Paper W358. p. 702.

Interpretive Summary:

Technical Abstract: Phosphorus (P) in the diets of animals in concentrated animal feeding operations (CAFOs) is of great importance with the increasing concern of environmental impact of animal agriculture. Excess phosphorus in diets of cattle is excreted in the manure and, if improperly managed, can be washed into local surface water causing an increase in algae growth, while a dietary deficiency can lead to poor growth and other detrimental symptoms. The objective of this study was to determine the expression of NaPi-IIb in the small intestine and parotid salivary gland and NaPi-IIa in the kidney of cattle fed increasing levels of P (0.29, 0.38, and 0.52 % P: 0, 30 and 60% wet distillers grain, respectively). Samples of parotid salivary gland and kidney along with the mucosa of the duodenum, proximal jejunum, distal jejunum, and ileum were collected at slaughter and immediately frozen in liquid nitrogen. Relative amounts of NaPi-IIa or NaPi-IIb mRNA were determined using RT-PCR. Expression of NaPi-IIa in the kidney was not affected by diet (P = 0.15). Expression of NaPi-IIb was highest in the ileum and proximal jejunum (P = 0.058). NaPi-IIb expression in the parotid and small intestine were also not affected by varying dietary P (P > 0.2). It appears that dietary P may play a lesser role in regulation of P transporter expression in ruminants than in other animal species.